The French National chemical library (Chimiothèque Nationale) offers a diverse collection of more 80,000 compounds and 15,000 original natural extracts. Packaged in a standardized form compatible with high- and medium-throughput screening, these compounds can be solicited in the framework of scientific collaborations, through simple, fast, inexpensive and legally protected procedures by a MTA (MTA_EN_GIS_CBF.pdf).
The Chimiothèque Nationale also offers competent partners for hit-to-lead developments.Find the general rules of access to the substances in this following document : regles_generales_de_fonctionnement_CN_mar2023-en.pdf
The Chimiothèque Nationale (CN) was created in 2003 by the CNRS. In 2007, the CN took the legal form of a Scientific Interest Group (GIS), through an agreement signed by 25 research organizations and higher education establishments. Since 2019, the CN has been one of the 4 pillars of ChemBioFrance Research Infrastructure.
The objectives of the CN are:
- the backup and conservation of the synthesis compounds and natural extracts patrimony existing in french academic laboratories;
- scientific valorization of these collections held by each CN laboratory.
The missions of the CN are:
- to federate the French academic research laboratories wishing to promote the constitution and the scientific and industrial valorization of their collections of compounds and natural substances, in a format compatible with high-throughput screening;
- to create a global database of these collections, more complete and diverse than each of the collections considered separately;
- to promote the compound and natural extracts collections towards chemists and biologists, academic and industrial researchers with a screening perspective.
The Chimiothèque Nationale works as a network of laboratories, in which each team remains responsible for its molecules and substances, and can decide of their distribution. In becoming a member of ChemBioFrance, you will be able to:
To become a member, you will have to :
To carry out the first 2 points, ChemBioFrance can give you a technical, financial and administrative assistance. The material needs are limited to only a system of storage of samples (bulk or plate), coupled to a computerized management (CN can provide the software).
To join ChemBioFrance : please contact the manager of the pilar Chimiothèque Nationale: Dr Florence Mahuteau.
https://chembiofrance.org/acces-aux-bases-de-donnees-utilisateurs-enregistres
86,086 compounds (
65 335
in plate) on the March 28th 2024: This collection is available in 96
or 384 well plates at a concentration of 5 mM in DMSO. Distributed volumes are between
2.5 and 300 nL
in echo plates.The
participation fee starts from 0.30 € HT per well, shipping included.
The 1040 compounds in this selection reflect the chemical diversity of the complete compound library of the CN. The quality of the compounds was checked in 2018 and all compounds are at least 80% pure. This collection is available in 96 or 384 well plates at a concentration of 5 mM in DMSO. The distributed volumes are between 1 and 3 µL in frozen standard plates. The stability of these compounds in solution in DMSO was also checked. The participation fee to acquire the CNE is 2211 € HT, shipping costs included.
Project request
It is possible to select only a subset of the National Chemical Library and to receive it in 96 or 384-well frozen plates. The selection of this subset can be achieved through molecular descriptors accessible online on the CN website or through chemoinformatic tools (analog search, virtual screening, etc ...). The cost contribution depends on the quantity of wells ordered and the packaging selected. From November 4th, 2020, a quote including the cost contribution will be provided following a cherry-picking (For ex., the cost of a microplate of 96 products is about 400€).
To download all structures from CN, please go the page "Access to the database for registered users only". To visualize and analyse data from the Sdfile, you could use DataWarrior (DataWarrior).
To request compounds from Chimiotheque Nationale by cherry-picking, you can follow this help guide : https://chembiofrance.org/faq
Project request
The
compounds of the library have been annotated according to their belonging to
chemical families such as "kinase-like", "GPCR-like", "iPPI-like", etc., in order to facilitate the selection targeted
libraries.
It
is possible to prepare plates containing concentration ranges, for example to
determine dose-response curves. The number of points (~ 10), the concentration
range and the volume of the wells have to be defined according to the experimental
test. The cost of the wells depends on the quantity of wells ordered.
Fr-PPIChem is a chemical library of 10,314 molecules dedicated to the inhibition of proteinprotein interactions (Bosc et al., 2020). These compounds are plated in a standardized form compatible with high or medium throughput screening.
The chemical library is the result of a research program involving 5 academic teams, national platforms and an industrial partner. The members of this consortium have developed two databases dedicated to protein-protein interaction modulators (http://2p2idb.inserm.fr and http://www.ippidb.cdithem.fr). The analysis of these databases has made it possible to define a physicochemical and structural profile characteristic of the inhibitors known to date, by comparing them to drugs or enzyme inhibitors (traditional targets) used in the Western pharmacopoeia.
Compounds with properties similar to protein-protein modulators were selected from catalogues of commercially available molecules using different supervised learning approaches. Physicochemical filters were applied to improve the ADME properties of the chemical library.
The cytotoxicity of the compounds was measured at two concentrations on 3 normal and cancer human cell lines by Dr. C. Barette (CEA-Grenoble, Caroline.Barette@cea.fr).
This chemical library can be requested from Dr Xavier Morelli (xavier.morelli@inserm.fr, CRCM, Marseille), coordinator of the national consortium or from Dr Marie-Odile Fauvarque (marie-odile.fauvarque@cea.fr, CMBA, Grenoble). The chemical library is provided in 384-well plates (33 plates in total) at a concentration of 10mM and a volume of 3 µl per well. Laboratories applying for the chemical library will have to indicate the objectives of the project and show their ability to screen on a miniaturized assay.
The consortium can propose qualified partners for the screening of this chemical library and the hit-to-lead developments of the identified hits.
Structures of the compounds in the chemical library are not available for download. However, the structures corresponding to the identified hits (as well as supplier information) will be sent in SDF format in return of the screening results.
Fr-PPIChem : Une chimiothèque dédiée aux Interactions Protéine-Protéine.
Contact : Xavier Morelli (xavier.morelli@inserm.fr)
Project requestA large collection (15116 on July 24th 2018) of natural extracts (plants, sponges, etc.) is available in frozen 96-well plates, in standard solutions (DMSO, 10 mg / mL). The whole collection is avalaible for screening.
Project requestYou may select the extracts from one or several botanic families. Like for the global library, the selected extracts are provided in frozen 96 wells microplates, in standard solution (DMSO, 10 mg/mL).
Project request