Target nature :
human DHODH
CAS n° :
-
Mecanism of action :
inhibition of the human DHODH catalytic activity (probably targeting the ubiquinone-binding pocket)
Physico-chemical properties :
- Molecular weight: 238.29 g/mol
- Experimental solubility: PBS pH7.4: 18 ± 1 µM; PBS pH7.4 10% DMSO: 57 ± 4 µM; NaCl 9/1000 with 10% cremophor EL: 6187 ± 17 µM; NaCl 9/1000 with 10% solutol HS15: 10919 ± 26 µM
- PK upon request
Probe availability :
upon request
Princeps reference :
Lucas-Hourani et al., 2013, PLoS Pathog 9(10): e1003678.
More bibliography :
Vidalain, P. O. et al., 2015, Medecine sciences : M/S. 31, 98-104; Grandin et al., 2016, Antiviral Research 125, 58-62.
Other available information on the probe :
- GAC50 (or ICC44133779) is an analog of DD264 (see dedicated page on the biotools list, DD264 has a chlorine atom instead of a vinyl), which was identified through a phenotypic screening to identify stimulators of ISRE-regulated genes.
- Metabolic stability on human liver microsomes at 37°C: t1/2 = 11.3 min
Other information on the target :
see review Munier-Lehmann et al., 2013, J. Med. Chem. 56(8), 3148-3167
Selectivity profile :
NA
In vivo data :
- GAC50 (administered topically with an intranasal sprayer) did not show any inhibitory effect against hRSV infection in macaques
In vitro data :
- inhibition of measles virus (IC50 = 1 µg/mL) and hRSV growth (IC50 = 25 µM); GAC50 is only active on primate cells.
Toxicity :
- Cell viability assay (CellTiterGlo reagent): CC50 > 250 µM (Hep2 and LLC-MK2 cells)
Chaine SMILES :
C=Cc3cccc(c1coc2CCCC(=O)c12)c3
Contact the author of the biotool
